Professor Choi’s team at SNU establishes the foundation for next-generation study to control Vibrio 바카라 커뮤니티

Professor Choi’s research team at SNU has identified the Vibrio 바카라 커뮤니티 transcription factors at a genome-wide scale and characterized their functions comprehensively, proposing novel targets to control the pathogen.

For successful infection, the life-threatening pathogen Vibrio 바카라 커뮤니티 elaborately regulates the expression of survival and virulence genes using various transcription factors (TFs).
In this study, a library of the V. 바카라 커뮤니티 mutants carrying specific signature tags in 285 TF genes was constructed and subjected to 16 phenotypic analyses. Consequently, 89 TFs affecting more than one phenotype of V. 바카라 커뮤니티 were identified.
Of these, 59 TFs affect the in vitro survival including growth, stress resistance, biofilm formation, and motility, and 64 TFs affect the virulence of V. 바카라 커뮤니티.
Particularly, 27 of the 64 TFs enhanced the in vitro hemolytic or cytotoxic activities, and 8 of the 27 TFs also increased the in vivo brine shrimp or murine infectivities of V. 바카라 커뮤니티. Among the 8 TFs, HlyU, IscR, NagC, MetJ, and Tet2 did not affect the growth of V. 바카라 커뮤니티 but still regulated the expression of major exotoxin genes, including rtxA, vvhA, and plpA, thereby emerging as potential drug targets for anti-virulence therapies with low selective pressure for developing resistance. Altogether, this study characterized the functions of TFs at a genome-wide scale and identified novel targets to control the virulence of V. 바카라 커뮤니티.